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发布于:2019-1-17 17:29:19  访问:11 次 回复:0 篇
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Hen derived two other populations (M and F) from each and every of
All these populations are maintained as 2-week discrete generation cycle, beneath 12 hrs:12 hrs L/D cycle at 25 (?) temperature and 60-80 relative 1082744-20-4 chemical information humidity. Typical corn meal-molasses-Yeast media is used to preserve them. The maintenance protocol is provided in Figure four. 1012104-68-5 Approximately 140?60 eggs are cultured in 8-10 mlof corn meal media per 8-dram vial (25 mm diameter ?90 mm height), referred to as juvenile competition vials. For each and every population, virgin males and females are collected beneath light CO2 anaesthesia on 10th day (post eclosion). All flies are collected for the duration of the peak of eclosion and held as single sex vials in the density of 8 per food-vial. Two days later, on 12th day (post egg collection), sexes PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/28250575 are combined following the selection regime ?M (24males: 8females in each vial), C (16male: 16female in each and every vial) and F (8male: 24female in each and every vial). Mixture was completed in fresh meals vials provisioned with measured quantity of live Yeast. Quantity of Yeast out there per female was kept continuous across the three selection regime at 0.47 mg / female. These vials (adult competitors vials) are kept undisturbed for two days and on 14th day on the cycle, the flies are transferred to fresh food vials (oviposition vials) and permitted to oviposit for 18 hours. Right after this 18 hour window, flies are discarded and the egg density is trimmed to 140?60 per vial, beginning the subsequent generation. Therefore the oviposition vials of one particular generation develop into the juvenile competitors vials with the subsequent generation. The powerful population sizes of allEggs cultured at a density 150 / 810ml meals per vialAdults commence eclosing from pupae on 10th dayMales and females are held as virgins in single sex vials (8individuals /vial) for two daysThe sexes interact for two daysFemales were permitted to oviposit for 18 hours in vials having 8-10ml foodOn 14th day post egg collection, flies are transferred to oviposition vials Pre-adult development requires about 10 days Eclosing flies are collected below CO2 anaesthesia as <6 hours old virgins On 12th day, post egg collection, sexes are combined in Yeasted fresh vials (32 individuals / vial). Sex ratio is in these vials correspond to the selection regime.Flies are discardedEgg density in these vials are trimmed to around 150/vial to start the next generationFigure 4 Maintenance protocol for the selection regimes. All the populations are maintained under 25 , 60 RH, 12 h:12 h Light/Dark cycle on standard cornmeal-molasses-Yeast media in 14-day discrete generation cycles. Larval density is controlled at approximately 150 per vial (8-10 ml of media). On 10th day post egg collection, adults are collected (using CO2 anaesthesia) and held as virgins (8 per vial). Two days later adults are combined in `yeasted‘ vials in the sex ratio corresponding to the respective selection regime. After two days of interaction period, flies are transferred to fresh food vials for oviposition and allowed an oviposition window of 18 hours.Hen derived two other populations (M and F) from each and every of C populations.
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